Description_x000D_ General description_x000D_ Mouse monoclonal antibody cocktail that contains a mixture of 3 antibodies supplied as undiluted ascites. Recognizes the ~50 kDa glial fibrillary acidic protein._x000D_ Recognizes ~50 kDa glial fibrillary acidic protein (GFAP) in human and bovine cytoskeletal preparations._x000D_ This Anti-Glial Fibrillary Acidic Protein Cocktail Mouse mAb is validated for use in ELISA, Frozen Sections, WB, ICC, Paraffin Sections for the detection of Glial Fibrillary Acidic Protein._x000D_ Immunogen_x000D_ Bovine_x000D_ purified bovine GFAP protein_x000D_ Application_x000D_ _x000D_ _x000D_ _x000D_ ELISA (1:1000)_x000D_ Frozen Sections (1:1000, see comments)_x000D_ Immunoblotting (1:1000)_x000D_ Immunocytochemistry (1:1000, see comments)_x000D_ Paraffin Sections (1:1000, trypsin or heat pre-treatment required)_x000D_ Physical form_x000D_ Undiluted ascites._x000D_ Reconstitution_x000D_ Upon initial thaw, aliquot and freeze (-20°C)._x000D_ Packaging_x000D_ 100 μL in Plastic ampoule_x000D_ Warning_x000D_ Toxicity: Standard Handling (A)_x000D_ Analysis Note_x000D_ Positive Control_x000D_ Astrocytes or cytoskeletal preparations_x000D_ Other Notes_x000D_ This cocktail is derived from the Bigner-Eng clones MAb1B4, MAb2E1, and MAb4A11 and provides a means for more comprehensive detection of astrocytomas than each clone alone. Each component is specific for GFAP and stains astrocytes and astrocytic processes as well as Bergman glia. Recognizes both anaplastic and reactive astrocytes by immunocytochemical staining. Does not recognize metastatic tumors and brain tumors of non-astrocytic origin, including medulloblastomas, meningiomas, choroid plexus papillomas, and schwannomas. For staining paraffin sections it is recommended that de-paraffinized sections be treated with 0.1% trypsin in 50 mM Tris-HCl, pH 7.6 for 20-30 min at 37°C or boiled in Tris-buffered saline, pH 9.0 for 15 min to expose the epitope. For immunocytochemistry or staining frozen sections, post-fixation in cold methanol or methanol/hydrogen peroxide for 10 min is required for access to the astrocytes in the sample. Antibody should be titrated for optimal results in individual systems._x000D_ Vick, W.W., et al. 1987. Acta. Cytol.31, 816._x000D_ McLendon R.E., et al. 1986. J. Neuropathol. Exp. Neurol.45, 692._x000D_ Pegram, C.N., et al. 1985. Neurochem. Pathol.3, 119.