Description General description Anti-Glucose Transporter-3, rabbit polyclonal, recognizes the ~45 kDa GLUT-3 protein. It is validated for use in ELISA, WB, IF, IP, and IHC (frozen sections). Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~45 kDa Glut-3 protein. Recognizes the ~45 kDa GLUT-3 protein. Immunogen a synthetic peptide corresponding to amino acids near the C-terminus of human GLUT-3, conjugated to KLH Application ELISA (0.5-1 µg/ml) Immunoblotting (1-10 µg/ml) Frozen Sections (2-20 µg/ml) Immunofluorescence (2-20 µg/ml) Immunoprecipitation (see comments) Packaging 50 μg in Glass bottle Please refer to vial label for lot-specific concentration. Warning Toxicity: Standard Handling (A) Other Notes Does not cross-react with other Glut isoforms. This antibody has also been reported to work for immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution. Mueckler, M. 1994. Eur. J. Biochem. 219, 713. Brant, A.M., et al. 1992. Biochem. Soc. Trans. 20, 236S. Nagamatsu, S., et al. 1992. J. Biol. Chem. 267, 467. Gould, G.W., et al. 1992. Diabetologia 35, 304. Kayano, T., et al. 1988. J. Biol. Chem. 263, 15245.