Description General description Anti-Glucose Transporter-4, rabbit polyclonal, recognizes the ~40-43 kDa GLUT-4. It is validated for use in ELISA, Western blotting, immunoprecipitation, and immunohistochemistry on frozen sections. Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~40-43 kDa Glut-4 protein. Recognizes the ~40-43 kDa GLUT-4 protein. Immunogen Mouse a synthetic peptide corresponding to amino acids near the C-terminus of mouse GLUT-4, conjugated to KLH Application ELISA (0.5-1 µg/ml) Frozen Sections (2-20 µg/ml) Immunoblotting (1-10 µg/ml; see comments) Immunoprecipitation (1-10 µg/100 µg tissue membrane) Physical form In PBS, 0.1% BSA, pH 7.5. Reconstitution Following initial thaw, aliquot and freeze (-20°C). Packaging 50 μg in Plastic ampoule Please refer to vial label for lot-specific concentration. Warning Toxicity: Standard Handling (A) Other Notes Does not cross-react with Hep G2-type transporter or other Glut isoforms. Important: Do not heat samples prior to immunoblotting, as heating will result in protein aggregation. Variables associated with assay conditions will dictate the optimal working dilution. Mueckler, M. 1994. Eur. J. Biochem. 219, 713. James, D.E., et al. 1989. Nature 338, 83. James, D.E., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 8368.