Description_x000D_ General description_x000D_ Anti-Glutathione-S-Transferase (GST) antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the hybridoma GST-2 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a purified recombinant GST fusion protein. Glutathione-S-Transferase (GST) is a 26 kDa protein found in eukaryotic cells. It belongs to the phase II detoxification enzymes family._x000D_ Immunogen_x000D_ purified recombinant GST fusion protein_x000D_ Application_x000D_ Anti-Glutathione-S-Transferase (GST) antibody, Mouse monoclonal has been used in:_x000D_ • immunoblotting_x000D_ • immunoprecipitation_x000D_ • dot blotting_x000D_ • autoradiography_x000D_ • immunofluorescence_x000D_ • enzyme-linked immunosorbent assay (ELISA)_x000D_ Physical form_x000D_ Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide._x000D_ Disclaimer_x000D_ Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals._x000D_ Biochem/physiol Actions_x000D_ Glutathione-S-Transferase (GST) helps to catalyze the conjugation of electrophilic substrates to reduced form of glutathione (GSH). Genetic engineering uses glutathione-S-transferase (from parasitic helminth Schistosoma japonicum) to create the GST gene fusion system for production of GST-labeled recombinant proteins. GST-fusion proteins were successfully demonstrated in structure- function studies involving protein-protein and DNA protein interactions via GST pull down assays.