Description_x000D_ General description_x000D_ Anti-Histone Deacetylase 2 (HDAC2) antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the HDAC2-62 hybridoma produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to the C-terminal region of human histone deacetylase 2 (amino acid residues with N-terminal added cysteine) conjugated to maleimide-activated KLH. Histone deacetylase 2 (HDAC2) is the catalytic subunits of different multiprotein regulatory complexes._x000D_ Specificity_x000D_ Monoclonal Anti-Histone Deacetylase 2 (HDAC2) recognizes human, bovine, dog, rat, mouse, and chicken HDAC2 (approx. 55 kDa). The antibody epitope resides within amino acids 471-488 of human HDAC2._x000D_ Application_x000D_ Anti-Histone Deacetylase 2 (HDAC2) antibody, Mouse monoclonal has been used in:_x000D_ • co-immunoprecipitation_x000D_ • chromatin immunoprecipitation (ChIP)_x000D_ • western blot_x000D_ • immunohistochemistry_x000D_ • enzyme linked immunosorbent assays (ELISA)_x000D_ Physical form_x000D_ Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide._x000D_ Disclaimer_x000D_ Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals._x000D_ Packaging_x000D_ 200 μL in glass insert_x000D_ Biochem/physiol Actions_x000D_ Histone Deacetylases including HDAC′s 1-10 facilitate transcriptional repression and nucleosome condensation. Abnormal recruitment of HDACs by various oncoproteins may occur in certain neoplastic diseases. Inhibition of HDAC2 activity by valproic acid induces proteasomal degradation of HDAC2.