Description_x000D_ General description_x000D_ Acetylation of lysine residues in proteins has emerged as an important mechanism in regulation and coordination of normal and pathophysiological cell metabolism. Lysine HMGylation is a newer post-translational protein modification that occurs under normal basal conditions. In murine models of HMG-CoA lyase deficiency, higher levels of protein lysine HMGylation has been observed. This rabbit polyclonal antibody specifically detects chemically modified proteins containing hydroxymethylglutaryl-lysine modifications, but not unmodified proteins. (Ref.: Wagner, GR et al. (2017). Cell Metabolism 25(4); 823-837)._x000D_ Specificity_x000D_ This rabbit polyclonal antibody detects BSA-conjugated 3-hydroxy-3-methyl-glutaryl (HMG)-Lysine. It recognizes BSA chemically modified to contain HMG-lysine modifications, but not unmodified BSA._x000D_ Immunogen_x000D_ 3-hydroxy-3-methyl-glutaryl (HMG)-Lysine conjugated to BSA._x000D_ Epitope: unknown_x000D_ Application_x000D_ Anti-HMG-Lysine, Cat. No. ABS2108, is a rabbit polyclonal antibody that detects hydroxymethylglutaryl-lysine conjugated proteins and has been tested for use in Western Blotting._x000D_ Research Category_x000D_ Signaling_x000D_ Western Blotting Analysis: A representative lot detected HMG-Lysine in Western Blotting applications (Wagner, G.R., et. al. (2017). Cell Metab. 25(4):823-837; Anderson, K.A., et. al. (2017). Cell Metab. 25(4):838-855.e15)._x000D_ Western Blotting Analysis: A 1:500 dilution from a representative lot detected HMG-Lysine in HMG-BSA conjugate (Courtesy of Dr Kristin Anderson from Duke University)._x000D_ Target description_x000D_ ~75 kDa observed (BSA conjugate). Uncharacterized bands may be observed in some lysate(s)._x000D_ Physical form_x000D_ Affinity Purified_x000D_ Format: Purified_x000D_ Purified rabbit polyclonal antibody in PBS with 0.1% sodium azide._x000D_ Storage and Stability_x000D_ Stable for 1 year at 2-8°C from date of receipt._x000D_ Disclaimer_x000D_ Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals._x000D_ Quality_x000D_ Evaluated by Western Blotting with HMG-BSA conjugate._x000D_ Western Blotting Analysis: A 1:500 dilution of this antibody detected HMG-Lysine in HMG-Lysine-BSA conjugate._x000D_ Other Notes_x000D_ Concentration: Please refer to lot specific datasheet.