Description General description Anti-Hsp70, mouse monoclonal, clone C92F3A-5, recognizes the ~70 kDa Hsp70. Does not cross-react with Hsc70. It is validated for use in ELISA, FC, WB, ICC, IP & IHC (frozen and paraffin sections). Mouse monoclonal antibody purified by ion-exchange chromatography. Recognizes the ~70 kDa Hsp70 protein. Recognizes the ~70 kDa Hsp70 protein. Does not cross-react with Hsc70. Immunogen Hsp70 from HeLa cells Human Application ELISA (see comments) Flow Cytometry (see comments) Frozen Sections (5 µg/ml) Immunoblotting (1 µg/ml) Immunocytochemistry (5 µg/ml) Immunoprecipitation (see comments) Paraffin Sections (5 µg/ml) Physical form In PBS, 50% glycerol, pH 7.2. Reconstitution Following iniital thaw, aliquot and freeze (-20°C). Packaging 50 μg in Plastic ampoule Please refer to vial label for lot-specific concentration. Warning Toxicity: Standard Handling (A) Analysis Note Positive Control L929 cells, Human colon cancer tissue Other Notes Does not cross-react with Hsc70. Because Hsp70 is not expressed constitutively in most cells, this antibody is suitable for ascertaining whether a stress-response has occurred in the cell. Increased levels of Hsp70 expression occurs following stress even in cells that constitutively express Hsp70. This antibody has also been reported to work for ELISA, flow cytometry, and immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution. Hang, H., and Fox, M.H. 1995. Cytometry19, 119. Kilgore, J.L., et al. 1994. J. Appl. Physiol.76, 589. Heufelder, A.E., et al. 1992. J. Clin. Endocrinol. Metab.74, 724. Gower, D.J., et al. 1989. J. Neurosurg.70, 605. Milarski, K., et al. 1989.J. Cell Biol.108, 413. Vass, K., et al. 1988. Acta Neuropathologica77, 128. Welch, W.J., and Suhan, J.P. 1986. J. Cell Biol.103, 2035.