Description_x000D_ General description_x000D_ Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. IgG is usually found as a monomer. IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. About 70 percent of the total immunoglobulin consists of IgG. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. Anti-Human IgG (Fc specific)-Alkaline Phosphatase antibody is specific for human IgG and human IgG, Fc fragment. The product does not react with human IgG, Fab fragment, light chains, IgA, and IgM. Additionally, the antibody does not bind to mouse and rat IgG. Goat anti-human IgG is conjugated to alkaline phosphatase by protein cross linking with 0.2% glutaraldehyde._x000D_ Specificity_x000D_ Specificity of the Anti-Human IgG is determined prior to conjugation by ELISA. The conjugate is specific for human IgG and human IgG, Fc fragment. Cross reactivity of the antibody-conjugate is also determined by ELISA. The conjugate shows no reactivity with human IgG, Fab fragment, human light chains, human IgA, human IgM or with mouse IgG and rat IgG._x000D_ Binds human IgG; does not bind other human Igs._x000D_ Provides reduced background with mouse or rat samples._x000D_ Immunogen_x000D_ Human IgG, Fc fragment_x000D_ Application_x000D_ A modified monoclonal antibody specific immobilization of platelet antigens (MAIPA) assay was performed on human platelet eluates using alkaline phosphatase-conjugated goat anti-human, Fc specific, antibody as the secondary with a 30 minute incubation._x000D_ Anti-Human IgG (Fc specific)-Alkaline Phosphatase antibody is suitable for use in ELISA and western blot at 1:5000 dilutions. The product can also be used for dot blot (1:40,000) and immunohistochemistry (1:150 using formalin-fixed, paraffin-embedded sections)._x000D_ Anti-Human IgG (Fc specific)-Alkaline Phosphatase antibody has been used in_x000D_ • quantitative sandwich ELISA_x000D_ • α-GalC ELISA_x000D_ • ELISA assays_x000D_ • ELISAs for IgG subclasses_x000D_ Physical form_x000D_ Solution in 0.05 M Tris, pH 8.0, containing 1% BSA, 1 mM MgCl2 and 15 mM sodium azide_x000D_ Storage and Stability_x000D_ Store at 2-8 °C. Do Not Freeze._x000D_ Disclaimer_x000D_ Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals._x000D_ Biochem/physiol Actions_x000D_ Immunoglobulin G (IgG) regulates immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders. Immunoglobulin G (IgG) mainly participates in hypersensitivity type II and type III._x000D_ Other Notes_x000D_ No cross-reaction with mouse and rat IgG._x000D_ Working dilutions should be determined by titration assay. Due to differences in assay systems, these titers may not reflect the user′s actual working dilution.