Description_x000D_ General description_x000D_ Anti-Human Lambda Light Chains (Bound and Free)- Peroxidase antibody, Mouse monoclonal (mouse IgG2a isotype) is derived from the HP-6054 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with purified human IgG myeloma proteins covalently coupled to polyaminostyrene (PAS) microbeads. Immunoglobulins are composed of two heavy and two light polypeptide chains held together by noncovalent forces and usually by interchain disulfide bridges. The various types of human (and other mammalian) immunoglobulins contain one of the two existing light chain types, either κ or λ in which multiple structural differences are reflected in antigenic variety, mainly the N-terminal (variable) domain of the chains._x000D_ Immunogen_x000D_ Purified human IgG myeloma proteins covalently coupled to polyaminostyrene (PAS) microbeads._x000D_ Application_x000D_ Direct ELISA: a working dilution of 1:16,000-1:32,000 is recommended using 2 μg/mL human IgG lambda light chains for coating._x000D_ Biochem/physiol Actions_x000D_ In monoclonal disorders, such as myeloma and macroglobulinemia, an increase in the level of a single immunoglobulin class can be accompanied by disproportionate increase in either λ or κ light chains. In many cases of B cell malignancy there is an increased production of light chains which are not accompanied with heavy chains. These circulate in the various body fluids (blood, cerebral spinal fluid, and tissues) and are also found in enormous amounts as free molecules in the urine (Bence Jones paraproteins)._x000D_ Physical form_x000D_ Supplied as a lyophilized powder._x000D_ Other Notes_x000D_ In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test.