Description General description Immunoaffinity purified mouse monoclonal antibody. Recognizes the ~85 kDa IKKα protein. Recognizes the ~85 kDa IKKα protein in Daudi cells. This Anti-IKKα Mouse mAb (14A231) is validated for use in Immunoblotting for the detection of IKKα. Immunogen Human a recombinant protein consisting of full-length human IKKα/IKK1 fused to a His•Tag sequence Application Immunoblotting (1-2 µg/ml, chemiluminescence) Physical form In PBS, 0.2% gelatin, pH 7.4. Reconstitution Following initial thaw, aliquot and freeze (-20°C). Packaging 100 μg in Plastic ampoule Please refer to vial label for lot-specific concentration. Warning Toxicity: Standard Handling (A) Analysis Note Positive Control Daudi cells Other Notes Antibody should be titrated for optimal results in individual systems. Zandi, E., et al. 1998 Science281, 1360. Didonato, J.A., et al. 1997 Nature388, 548. Lee, F.S., et al. 1997 Cell88, 213. Mercurio, F., et al. 1997 Science278, 860. Regnier, C.H., et al. M. 1997 Cell90, 373. Verma, I. and Stevenson, J.K. 1997 Proc. Nat. Acad. Sci. USA94, 11758. Verma, I.M., et al. 1995 Genes. Dev.9, 2723.