Description_x000D_ General description_x000D_ The gene LC3, also referred to as MAP1LC3A (microtubule associated protein 1 light chain 3 α), is a homologue of Apg8p necessary for autophagy in yeast. The cytosolic form upon processing gets localized to the autophagosome membranes. The gene is mapped to human chromosome 20q11.22. LC3 exists in cells as cytoplasmic LC3-I (18kDa) and LC3-II (16kDa)._x000D_ Immunogen_x000D_ Synthetic peptide corresponding to amino acids of human LC3A isoform a, conjugated to KLH via C-terminal cysteine residue. The corresponding sequence is identical in rat and mouse._x000D_ Application_x000D_ Anti-LC3 antibody produced in rabbit has been used in:_x000D_ • western blotting_x000D_ • immunofluorescence_x000D_ • immunohistochemistry_x000D_ • filter retardation assay_x000D_ • immunocytochemistry_x000D_ Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below._x000D_ Immunofluorescence (3 papers)_x000D_ Immunohistochemistry (1 paper)_x000D_ Western Blotting (21 paper)_x000D_ Physical form_x000D_ Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide._x000D_ Storage and Stability_x000D_ For continuous use, store at 2-8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours._x000D_ Disclaimer_x000D_ Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals._x000D_ Biochem/physiol Actions_x000D_ The gene MAP1LC3A (microtubule associated protein 1 light chain 3 α) encodes a protein that serves as a marker of autophagosomes. It is found to be expressed on surviving oligodendrocytes in Nasu-Hakola disease brains and may be involved in the induction of oligodendrogliopathy.