Description General description Purified mouse monoclonal antibody. Recognizes the Lewis y blood group antigen. Recognizes the Lewis y carbohydrate chain in colon adenocarcinoma. This Anti-Lewis y Mouse mAb (F3) is validated for use in Immunohistochemistry for the detection of Lewis y. Immunogen Human human SK-LU-3 lung cancer cells Application Immunohistochemistry (1:10-1:120) Physical form In PBS, 1% BSA. Reconstitution Following initial thaw, aliquot and freeze (-20°C). Packaging 1 ml in Plastic ampoule Please refer to vial label for lot-specific concentration. Warning Toxicity: Standard Handling (A) Analysis Note Positive Control Colon adenocarcinoma tissue Other Notes Cordon-Cardo, C., et al. 1986. Int. J. Cancer37, 667. Sakamoto, J., et al. 1986. Cancer Res. 46, 1553. Sakamoto, J., et al. 1984. Mol. Immunol. 21, 1093. For immunohistochemistry, fix tissue in 10% formalin buffered to neutral pH. Variables associated with assay conditions will dictate the proper working dilutions.