Description General description MUC18 antigen (CD146), a member of the immuoglobulin superfamily, is an intrinsic membrane glycoprotein of 110-120 kDa found on the surface of endothelial cells, bone marrow fibroblasts and various melanomas. MUC18 has been used as a marker of tumor progression in human melanoma because expression in those tumors correlates strongly with poor prognosis and the development of metastic disease. In addition, a number of human T, B and myeloid leukemic cell lines seem to express MUC18. The close structural relationship with N-CAM and related molecules suggests that MUC18 may be also a developmentally regulated cell adhesion molecule (Melanoma adhesion molecule or MCAM). Specificity Detects circulating endothelial cells in human. Expected to cross-react with dog & mouse. Immunogen Immunization of mice with HUVEC. Application Anti-MCAM Antibody, clone P1H12 detects level of MCAM & has been published & validated for use in ELISA, FC, IC, IH, IP & WB. Immunocytochemistry: 1-10 μg/mL of a previous lot worked in immunocytochemistry.Works best on EDTA or Trypsin lifted endothelial cells. Immunohistochemistry: 1-10 µg/mL. 4% PFA for 30min RT or <2hrs at 4°C. Block w/ 1%BSA/0.2% tween20/PBS for 30min. Works well in frozen tissue; fixed or unfixed. Immunoprecipitation: 1-10 μg/mL of a previous lot worked in immunoprecipitation. ELISA: 1-10 μg/mL of a previous lot worked in ELISA. FACS Analysis: 1-10 μg/mL of a previous lot worked in FACS. Optimal working dilutions must be determined by end user. Target description 110 kDa Physical form Format: Purified Purified mouse monoclonal IgG1 in buffer containing 0.02 M PB with 0.25 M NaCl, pH=7.6, with 0.1% sodium azide. Quality Routinely evaluated by Western Blot on HUVEC lysates. Western Blot Analysis: 1:500 dilution of this lot detected MCAM on 10μg of HUVEC lysates. Analysis Note Control HUVEC cells. Other Notes Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.