Description_x000D_ General description_x000D_ MIB1 (Mindbomb homology 1) are E3 ubiquitin ligases. It is abundantly expressed in both embryos and adult tissues, whereas MIB2 is highly expressed in adult tissues._x000D_ Mindbomb homolog 1 (MIB1) is an ubiquitin ligase which possesses two substrate recognition domains, ankyrin repeats and an amino terminal zinc finger domain._x000D_ Immunogen_x000D_ synthetic peptide corresponding to amino acids 924-939 of human MIB1, conjugated to KLH. The corresponding sequence is identical in rat and mouse._x000D_ Application_x000D_ Anti-MIB1 (C-terminal) antibody produced in rabbit has been used in:immunofluorescence, immunoprecipitation , western blotting immunohistochemistry_x000D_ Anti-MIB1 (N-terminal) antibody produced in rabbit has been used for immunofluorescence._x000D_ Physical form_x000D_ Solution in 0.01 M phosphate buffered saline, pH7.4, containing 15 mM sodium azide._x000D_ Disclaimer_x000D_ Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals._x000D_ Biochem/physiol Actions_x000D_ MIB1 is critical for mammalian development and cellular differentiation. It also plays a role in neuronal morphogenesis partially through physical interaction with cyclin-dependent kinase 5 (CDK5)/p35. Knockout of MIB1 in mice results in embryonic mortality with many defects observed in somitogenesis, neurogenesis, vasculogenesis and cardiogenesis._x000D_ Mindbomb homolog 1 (MIB1) aids in the ubiquitination and internalization of Notch ligands such as the protein δ. It also acts as a positive regulator in the activation of nuclear factor-κB (NF-κB).