Description_x000D_ General description_x000D_ MKLN1 (Muskelin 1, intracellular mediator containing kelch motifs) is a primarily cytoplasmic protein consisting of mainly two domains, N- and C-terminal domains. Specifically, it is composed of a discoidin-like domain and a LiSH/CTLH domain. The C-terminal domain consists of a kelch motif with 6 kelch repeats followed by a C-terminal tail._x000D_ Immunogen_x000D_ Muskelin recombinant protein epitope signature tag (PrEST)_x000D_ Application_x000D_ All Prestige Antibodies®Powered by Atlas Antibodies is developed and validated by the Human Protein Atlas (HPA) project (www.proteinatlas.org). Each antibody is tested by immunohistochemistry against hundreds of normal and disease tissues. These images can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. To view these protocols and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige._x000D_ Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below._x000D_ Immunohistochemistry (1 paper)_x000D_ Physical form_x000D_ Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide_x000D_ Legal Information_x000D_ Prestige Antibodies is a registered trademark of Sigma-Aldrich Co. LLC_x000D_ Biochem/physiol Actions_x000D_ MKLN1 (Muskelin 1, intracellular mediator containing kelch motifs) is mainly involved in different intracellular signaling pathways for scaffolding and nucleocytoplasmic protein shuttling. Its kelch repeats domain directly binds to the T-box DNA-binding domain of TBX20b isoform. It has been suggested that TBX20b and MKLN1 may be involved in novel regulatory mechanisms within heart development._x000D_ Features and Benefits_x000D_ Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section._x000D_ Every Prestige Antibody is tested in the following ways:_x000D_ • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues._x000D_ • Protein array of 364 human recombinant protein fragments._x000D_ • Validated for multiple commonly used applications such as IHC (Immunohistochemistry), IF (Immunofluorescence), and WB (Western Blot)_x000D_ Linkage_x000D_ Corresponding Antigen APREST74968.