Description_x000D_ General description_x000D_ Interleukins are proinflammatory cytokines produced on cell injury or trauma. There are two closely related types of interleukins produced by the cell, IL-1α and IL-1β that are produced by the activation of NF-κ B transcription factor in response to bacterial infection or LPS. Both IL-1α and IL-1β exert their cellular effects by signalling through IL-1 receptor type 1. A variety of cells such as macrophages, epithelial cells, fibroblasts, keratinocytes, monocytes and dendritic cells secrete IL-1α. Both IL-1α and IL-1β are important in acquired immunity, inflammation, response to infection and production of matrix metalloproteinases (MMPs). The effects of IL-1α are mediated by activation of NF-κB, JNK and MAPK pathways that activated the genes in response to infection and inflammation. Overproduction of IL-1α has been implicated in inflammatory disorders like rheumatoid arthritis and periodontitis_x000D_ Anti-interleukin-1α specifically reacts with mouse IL-1α. It does not react with human IL-1α and IL-1β or mouse IL-1β._x000D_ Specificity_x000D_ The antibody will neutralize the biological activity of recombinant mouse IL-1α, but not recombinant human IL-1α, IL-1β or recombinant mouse IL-1β._x000D_ Immunogen_x000D_ recombinant mouse IL-1α._x000D_ Application_x000D_ Anti-interleukin 1α antibody is suitable for neutralization reactions and the recommended ND50 is 0.04-0.2 μg/ml. The antibody may be used for immunoblotting at a working concentration of 1-2 μg/ml. It is also suitable for indirect ELISA applications._x000D_ Physical form_x000D_ Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline pH 7.4, with 5% trehalose._x000D_ Disclaimer_x000D_ Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.