Description General description The murine leukemia virus (MuLV) is a gammaretrovirus that has a positive, single-stranded sense RNA, which replicates via reverse transcription. The envelope of the virus is shown to be covered with glycoprotein spikes. Three major genes produced by the murine leukemia virus are gag, pol, and env. The gag gene codes for the group-specific antigen that is responsible for the production of the viral matrix capsid and nucleoproteins. The pol gene encodes reverse transcriptase, a protease, and an integrase. The reverse transcriptase is used to make complementary DNA by reverse transcribing its own RNA into DNA. The reverse transcriptase in MuLV is able to act as a monomer as opposed to a dimer. The integrase integrates the synthesized viral DNA into the host cell s DNA. The env gene codes for a glycosylated protein that is processed into the two viral envelope proteins, gp70 and p15(E), which aid the virus in selecting and attacking host cells. The gp70 protein has the receptor binding activity of the virus. Retrovirus membrane fusion is catalyzed by the Env, surface (SU) and transmembrane (TM) proteins. The SU protein is involved in receptor binding, while the TM protein contains the necessary elements for membrane fusion. Clone 42-114 is TM-specific and does not recognize G541R mutants. (Ref. Schneider, WM., et al. (2008). Virology. 371(1); 165-174). Specificity Clone 42-114 detects murine leukemia virus in infected cells. Immunogen AKR K36 and AKR SL3 leukemia virus. Application Anti-MuLV TM, clone 42-114, Cat. No. MABF2074, is a mouse monoclonal antibody that detects Murine leukemia virus (MuLV) transmembrane protein and has been tested for use in Immunoprecipitation and Western Blotting. Immunoprecipitation Analysis: A representative lot immunoprecipitated MuLV TM (Pinter, A., et. al. (1982). Virology. 116(2):499-516). Western Blotting Analysis: A representative lot detected MuLV TM in Western Blotting applications (Schneider, W.M., et. al. (2008). Virology. 371(1):165-74). Research Category Inflammation & Immunology Target description ~15 kDa observed. Uncharacterized bands may be observed in some lysate(s). Physical form Format: Purified Purified by ion-exchange chromatography Purified mouse monoclonal antibody IgM in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Storage and Stability Stable for 1 year at 2-8°C from date of receipt. Disclaimer Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. Quality Evaluated by Western Blotting in HEK293 cells infected with murine leukemia virus (MuLVs). Western Blotting Analysis: A 1:250 dilution of this antibody detected MuLV TM in HEK293 cells infected with murine leukemia virus (MuLVs). Other Notes Concentration: Please refer to lot specific datasheet.