Description_x000D_ General description_x000D_ _x000D_ ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies._x000D_ Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them._x000D_ Learn more about ZooMAb here._x000D_ Specificity_x000D_ Clone 1E15 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Myosin Heavy Chain 1. it targets an epitope within 18 amino acids from the N-terminal region._x000D_ Immunogen_x000D_ KLH-conjugated linear peptide corresponding to 18 amino acids from the N-terminal region of human Myosin Heavy Chain 1._x000D_ Application_x000D_ Anti-MYH1, clone 1E15 ZooMAb, Cat. No. ZRB1214, is a highly specific recombinant rabbit monoclonal antibody that detects Myosin heavy chain 1 (MYH1) and has been tested for use in Immunofluorescence, Immunohistochemistry (Paraffin) and, Western Blotting._x000D_ Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected MYH1 in C2C12 cell lysate._x000D_ Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected MYH1 in human skeletal muscle tissue sections._x000D_ Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected MYH1 in human skeletal muscle and mouse skeletal muscle tissue sections._x000D_ Note: Actual optimal working dilutions must be determined by end user as specimens and experimental conditions may vary._x000D_ Target description_x000D_ Myosin-1 (UniProt: P12882; also known as Myosin heavy chain 1, Myosin heavy chain 2x, MyHC-2x, Myosin heavy chain IIx/d, MyHC-IIx/d, Myosin heavy chain, skeletal muscle adult 1) is encoded by the MYH1 gene (Gene ID: 4619) in human. Myosin is a major contractile protein that converts chemical energy into mechanical energy through the hydrolysis of ATP. Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). Limited proteolysis of myosin heavy chain produces 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). HMM can be further cleaved into 2 globular sub-fragments (S1) and 1 rod-shaped sub-fragment (S2). In mammals at least 10 different myosin heavy chain (MYH) isoforms have been described from striated, smooth, and non-muscle cells. These isoforms show expression that is spatially and temporally regulated during development. Myosin-1 contains an N-terminal SH3-like domain (aa 33-82), a myosin motor domain (aa 86-785), and an IQ domain (aa 788-817). It also contains 2 actin binding regions (aa 662-684 and 764-778). This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals._x000D_ Physical form_x000D_ Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL._x000D_ Reconstitution_x000D_ 30 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type._x000D_ Storage and Stability_x000D_ Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws._x000D_ Disclaimer_x000D_ Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals._x000D_ Legal Information_x000D_ ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany