Description General description Purified mouse monoclonal antibody generated by immunizing BALB/c mice with specified immunogen and fusing splenocytes with SP2/0 mouse myeloma cells (see application references). Recognizes N-myc and its cleavage products. Recognizes the N-myc protein and its cleavage product in IMR5 cells. This Anti-N-Myc Mouse mAb (NCM II 100) is validated for use in Frozen Sections, Immunoblotting, Immunofluorescence, Immunoprecipitation for the detection of N-Myc. Immunogen Human recombinant, human, N-myc fusion protein, expressed in E. coli Application Frozen Sections (1-5 µg/ml) Immunoblotting (1-5 µg/ml, see application references) Immunofluorescence (1-5 µg/ml, see application references) Immunoprecipitation (1-2 µg/sample, see application references) Physical form In 50 mM sodium phosphate buffer, 50% glycerol. Reconstitution Following initial thaw, aliquot and freeze (-20°C). Packaging 100 μg in Plastic ampoule Please refer to vial label for lot-specific concentration. Warning Toxicity: Standard Handling (A) Analysis Note Positive Control IMR5 cells Other Notes Brondyk, W.H. 1991. Oncogene6, 1269. Ikegaki, N., et al. 1988. Adv. Neuroblastoma Research.2, 133. LeGouy, E., et al. 1987. In Nuclear Oncogenes, Cold Spring Harbor Laboratory, 144. Cole, M.D., 1986. Ann. Rev. Gen.20, 361. Ikegaki, N., et al. 1986. Proc. Natl. Acad. Sci. USA83, 5929. Slamon, D.J., et al. 1986. Science232, 768. Seeger, R.C., et al. 1985. New Engl. J. Med.313, 1111. Brodeur, G.M., et al. 1984. Science224, 1121. Michitsch, R.W., et al. 1984. Mol. Cell. Biol.11, 2370. Kohl, N.E., et al. 1983. Cell35, 603. Schwab, M., et al. 1983. Nature305, 245. It is important to compensate for the short half life of this protein when preparing tissue sections or extracts. We recommended that all preparations be kept cold and that a cocktail of protease inhibitors be used. Antibody should be titrated for optimal results in individual systems.