Description General description Rabbit polyclonal antibody. Recognizes the ~65 kDa NF-κB p65 (RelA) protein. Recognizes the ~65 kDa NF-κB p65 (RelA) protein in Daudi cells. This Anti-NF-κB (p65, RelA) (Ab-2) Rabbit pAb is validated for use in ELISA, Gel Shift, Immunoblotting, Immunoprecipitation for the detection of NF-κB (p65, RelA) (Ab-2). Immunogen Human a synthetic peptide corresponding to amino acids near the N-terminal domain of human NF-κB p65 (RelA), conjugated to KLH Application ELISA (see comments) Gel Shift (see comments) Immunoblotting (1:500, chemiluminescence) Immunoprecipitation (see comments) Physical form In 5 mM sodium phosphate buffer, pH 7.4. Reconstitution Following initial thaw, aliquot and freeze (-20°C). Packaging 100 μL in Plastic ampoule Warning Toxicity: Standard Handling (A) Analysis Note Positive Control Daudi cells Other Notes Kolberg, R., 1997. J. NIH Research9, 29. Van Antwerp, D.J. et al. 1996. Science274, 787. Beg, A.A., et al. 1995. Nature376, 167. Baeuerle, P.A., and Henkel, T., 1994. Ann. Rev. Immunol.12, 141. Wulczyn, F.G., et al. 1992. Nature358, 597. This antibody has also been reported to work for ELISA, gel shift, and immunoprecipitation. Antibody should be titrated for optimal results in individual systems.