Description_x000D_ General description_x000D_ NADPH:quinone oxidoreductase (NQO1) is an FAD containing electron reductase that regulates the detoxification of quinones obtained from the phenolic metabolites of benzene. It is a homodimeric protein that can bind either of the reducing cofactors; NADPH or NADH . NQO1 regulates chemoprotection, neuroprotection, antitumor quinones and antioxidative defense mechanisms . NQO1 may be overexpressed in breast, colon, pulmonary and hepatic tumors._x000D_ NADPH:quinone oxidoreductase (NQO1, DTdiaphorase) is a 2-electron reductase. NQO1 is a FAD-containing protein that exists as a homodimer. It is ubiquitously expressed._x000D_ Specificity_x000D_ Anti-NQO1 (C-terminal) antibody is specific for rat, mouse, and human NQO1. The immunizing peptide specifically inhibits the detection of NQO1 band by immunoblotting._x000D_ Immunogen_x000D_ Synthetic peptide sequence corresponding to amino acid residues 261-274 of human NQO1, with N-terminal added cysteine conjugated to KLH. The corresponding sequence differs by one amino acid in mouse and rat._x000D_ synthetic peptide corresponding to amino acid resudues 261-274 of human NQO1 with N-terminal added cysteine conjugated to KLH. The corresponding sequence differs by one amino acid in mouse and rat._x000D_ Application_x000D_ Anti-NQO1 (C-terminal) antibody may be used in immunological applications such as immunoprecipitation, immunofluorescence and immunoblotting (approximately 28 kDa)._x000D_ Anti-NQO1 (C-terminal) antibody produced in rabbit has been used in:_x000D_ • western blotting_x000D_ • immunoprecipitation_x000D_ • immunofluorescence_x000D_ Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below._x000D_ Western Blotting (1 paper)_x000D_ Physical form_x000D_ Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide._x000D_ Disclaimer_x000D_ Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals._x000D_ Biochem/physiol Actions_x000D_ NADPH:quinone oxidoreductase (NQO1, DTdiaphorase) can bioactivate certain antitumor quinones. NQO1 has a role in antioxidant defense via the generation of antioxidant forms of ubiquinone and vitamin E. High levels of NQO1 gene expression has been observed in liver and lung tumors as compared to normal tissues of the same origin.