Description General description Purified rabbit polyclonal antibody. Recognizes the ~230 kDa 53BP1 protein. Recognizes the ~230 kDa 53BP1 protein in HeLa cell nuclear extract (Cat. No. WB64). This Anti-p53 Binding Protein 1 (Ab-1) Rabbit pAb is validated for use in Immunoblotting, Immunofluorescence, Immunoprecipitation for the detection of p53 Binding Protein 1 (Ab-1). Immunogen Human a recombinant protein consisting of human 53BP1 fused to a His•Tag sequence Application Immunoblotting (1:3000-1:5000) Immunofluorescence (1:300-1:500, indirect) Immunoprecipitation (1:300-1:500) Physical form In 100 mM NaCl, 50 mM Tris-HCl, 20% glycerol, pH 7.6. Reconstitution Following initial thaw, aliquot and freeze (-70°C). Packaging 100 μL in Plastic ampoule Please refer to vial label for lot-specific concentration. Warning Toxicity: Standard Handling (A) Analysis Note Positive Control HeLa Cell Nuclear Extract (Cat. No. WB64) Other Notes Antibody should be titrated for optimal results in individual systems.