Description General description Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0-Ag14 mouse myeloma cells. Recognizes the ~37 kDa PCNA protein. Recognizes the ~37 kDa PCNA protein in HeLa cells as well as in tonsil or normal colon tissue. This Anti-PCNA (Ab-1) Mouse mAb (PC10) is validated for use in Immunoblotting, Immunofluorescence, Immunoprecipitation Paraffin Sections for the detection of PCNA (Ab-1). Immunogen Epitope: Within amino acids 112-121 recombinant PCNA Application Immunoblotting (2.5 µg/ml) Immunofluorescence (1-5 µg/ml) Immunoprecipitation (not recommended with unconjugated antibody, see comments) Paraffin Sections (2.5 µg/ml, no pre-treatment required, see comments) Packaging 200 μg in Plastic ampoule Please refer to vial label for lot-specific concentration. Warning Toxicity: Standard Handling (A) Analysis Note Positive Control HeLa cells, HL-60 cells, tonsil tissue, or normal colon tissue Other Notes Staining shows an intense nuclear pattern. Not recommended for immunoprecipitation because in some samples a ~37 kDa protein will be precipitated that is not PCNA. Generally, no pre-treatment is needed to stain paraffin sections, but staining may be enhanced in some samples by pretreating with 4 N HCl for 10 min at 37°C followed by 3 washes in water. Antibody should be titrated for optimal results in individual systems. Waseem, N.H. and Lane, D.P. 1990. J. Cell. Sci.96, 121. Suzuka, I., et al. 1989. Proc. Natl. Acad. Sci. USA86 3189. Bravo, R., et al. 1987. Nature (London)326, 515. Bravo, R. and MacDonald-Bravo, H. 1987. J. Cell. Biol.105, 1549. Miyachi, K., et al. 1987. J. Immunol.121, 2228.