Description_x000D_ Immunogen_x000D_ Immunogen Sequence: GI # 9885433, sequence 80-196_x000D_ Recombinant charged multivesicular body protein 1/chromatin modifying protein 1_x000D_ Application_x000D_ Anti-PCOLN3 antibody produced in chicken is suitable for western blotting analysis at a dilution of 1:500, for tissue or cell staining at a dilution of 1:200._x000D_ Physical form_x000D_ Solution in phosphate buffered saline containing 0.02% sodium azide._x000D_ Biochem/physiol Actions_x000D_ Charged multivesicular body protein 1a is a protein encoded by the CHMP1A gene in humans and is a member of the ′Endosomal Sorting Complex Required for Transport′ (ESCRT-III) family. It plays a role in the cytoplasm in sorting proteins to the multivesicular body (MVB) and functions as a tumor suppressor gene in renal cells. It may be a useful target for treatment of renal cell carcinoma (RCC). Overexpression of this gene induces growth inhibition, chromatin condensation and p53 phosphorylation. The gene regulates Ataxia telangiectasia mutated (ATM) and the nuclear localization signal (NLS) that is required for ATM activation. It also regulates tumor growth partly through ATM signaling. It is a novel tumor suppressor, especially in pancreas and regulates tumor growth potentially through p53 signaling pathway. It also plays a role in stable gene silencing within the nucleus.