Description_x000D_ General description_x000D_ Information PHD finger protein 1 (UniProt: O43189; also known as PHF1, hPHF1, Polycomb-like protein 1, hPCl1) is encoded by the PHF1 (also known as PCL1) gene (Gene ID: 5252) in human. PHF1 is a member of the polycomblike family and contains two PHD-type zinc fingers (aa 87-142 and 186-240) and one Tudor domain (aa 29-86). It is essential for epigenetic regulation and genome maintenance. It specifically binds histone H3 trimethylated at ′Lys-36′ (H3K36me3) via its Tudor domain and recruits the PRC2 complex. PHF1 is highly expressed in heart, skeletal muscle, and pancreas, with lower levels found in brain, placenta, lung, liver and kidney. It is involved in DNA damage response and is recruited at double-strand breaks. PHF1 localizes to the promoters of numerous target genes and to double-strand breaks (DSBs) sites following DNA damage. It has been reported that PRC2 recruitment by PHF1 promotes H3K27me3 and subsequent gene silencing by inducing spreading of PRC2 and H3K27me3 into H3K36me3 loci. A chromosomal aberration involving PHF1 is reported to be the causative factor in endometrial stromal tumors. (Ref.: Sarma, K et al. (2008). Mol. Cell. Biol. 28(8): 2718-31; Musselman, CA et al (2012). Nat. Struct. Mol. Biol. 19(12):1266-72)._x000D_ Specificity_x000D_ This rabbit polyclonal antibody detects Information PHD finger protein 1 (PHF1) in human cells. It targets an epitope within 137 amino acids from the internal region._x000D_ Immunogen_x000D_ GST-tagged recombinant fraction corresponding to 137 amino acids from the internal region of human PHD finger protein 1._x000D_ Application_x000D_ Anti-PHF1, Cat. No. ABE863, is a highly specific rabbit polyclonal antibody that targets PHD finger protein 1 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting._x000D_ Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected PHF1 in human pancreas and human kidney tissues._x000D_ Western Blotting Analysis: A 1:500 dilution from a representative lot detected PHF1 in 10 µg of HeLa cell lysate._x000D_ Research Category_x000D_ Epigenetics & Nuclear Function_x000D_ Target description_x000D_ ~65 kDa observed; 62.11 kDa calculated. Uncharacterized bands may be observed in some lysate(s)._x000D_ Physical form_x000D_ Affinity Purified_x000D_ Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide._x000D_ Storage and Stability_x000D_ Stable for 1 year at 2-8°C from date of receipt._x000D_ Disclaimer_x000D_ Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals._x000D_ Quality_x000D_ Evaluated by Western Blotting in HeLa cell nuclear extract._x000D_ Western Blotting Analysis: A 1:500 dilution of this antibody detected PHF1 in 10 µg of HeLa cell nuclear extract._x000D_ Other Notes_x000D_ Concentration: Please refer to lot specific datasheet.