Description General description Purified mouse monoclonal antibody. Recognizes the ~60 kDa PIP5K3 protein. Recognizes the ~60 kDa PIP5K3 protein in human testes tissue and HeLa cells. This Anti-PIP5K3 Mouse mAb (6C7) is validated for use in ELISA, Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of PIP5K3. Immunogen A recombinant polypeptide corresponding to amino acids 342-452 of human PIP5K3, expressed as a GST fusion protein Human Application ELISA (see comments) Immunoblotting (1-5 µg/ml) Immunocytochemistry (10 µg/ml) Paraffin Sections (3 µg/ml) Physical form In PBS, pH 7.2. Reconstitution Following initial thaw, aliquot and freeze (-20°C). Warning Toxicity: Regulatory Review (Z) Analysis Note Negative Control 293T cells Positive Control Human testes tissue, HeLa cells Other Notes For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution. Tsuruta, F., et al. 2009. J. Cell Biol.187 279.