Description_x000D_ General description_x000D_ Regulator of G-protein signaling 2 (UniProt: P41220; also known as RGS2, Cell growth-inhibiting gene 31 protein, G0/G1 switch regulatory protein 8) is encoded by the RGS2 (also known as G0S8, GIG31) gene (Gene ID: 5997) in human. RGS2 is shown to regulate G protein-coupled receptor signaling cascades. It inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form. It also plays a role in the regulation of blood pressure in response to signaling via G protein-coupled receptors and GNAQ. RGS2 is reported to play a role in regulating the constriction and relaxation of vascular smooth muscle and also has a protective effect against myocardial hypertrophy and atrial arrhythmias. It inhibits beta-adrenergic receptor-induced cardiomyocyte hypertrophy. Overexpression of RGS2 reduces the activity of isoproterenol-induced ERK1/2 and prevents AKT activation. Four isoforms of RGS2 have been reported that are produced by alternative splicing. (Ref.: Nunn, C et al. (2010). Cell Signal 22(8); 1231-1239)._x000D_ Specificity_x000D_ Clone 9D9.1 specifically detects Regulator of G-protein signaling 2 (RGS2) in human and rat cells. It targets an epitope within 82 amino acids from the N-terminal region._x000D_ Immunogen_x000D_ GST/His-tagged recombinant fragment corresponding to 82 amino acids from the N-terminal region of human Regulator of G-protein signaling 2 (RGS2)._x000D_ Application_x000D_ Anti-RGS2, clone 9D9.1, Cat. No. MABC1220, is a mosue monoclonal antibody that detects RGS2 in human and rat cells and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting._x000D_ Research Category_x000D_ Apoptosis & Cancer_x000D_ Western Blotting Analysis: 1 µg/mL from a representative lot detected RGS2 in 10 µg of HL-60 cell lysate._x000D_ Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected RGS2 in human cerebral cortex, human pancreas, human lymphoma, and rat testis tissues._x000D_ Target description_x000D_ ~25 kDa observed; 24.38 kDa calculated. Uncharacterized bands may be observed in some lysate(s)._x000D_ Physical form_x000D_ Format: Purified_x000D_ Protein G purified_x000D_ Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide._x000D_ Storage and Stability_x000D_ Stable for 1 year at 2-8°C from date of receipt._x000D_ Disclaimer_x000D_ Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals._x000D_ Quality_x000D_ Evaluated by Western Blotting in Raji cell lysate._x000D_ Western Blotting Analysis: 1 µg/mL of this antibody detected RGS2 in 10 µg of Raji cell lysate._x000D_ Other Notes_x000D_ Concentration: Please refer to lot specific datasheet.