Description_x000D_ General description_x000D_ Ras and Rab interactor 3 (RIN3) is a novel Rab5-binding protein, encoded by the gene mapped to human chromosome 14q32. The encoded protein belongs to the RIN family of RAS effectors. RIN3 contains a SH2 domain, proline-rich domain, RH domain, vacuolar protein sorting-associated protein (Vps9) domain and ras-associating (RA) domain in the N- terminal region. RIN3 show tissue-specific expression and is highly expressed in mast cells._x000D_ Immunogen_x000D_ Ras and Rab interactor 3 recombinant protein epitope signature tag (PrEST)_x000D_ Application_x000D_ Anti-RIN3 antibody produced in rabbit has been used in immunohistochemistry._x000D_ Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below._x000D_ Immunohistochemistry (1 paper)_x000D_ Physical form_x000D_ Solution in phosphate buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide._x000D_ Disclaimer_x000D_ Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals._x000D_ Legal Information_x000D_ Prestige Antibodies is a registered trademark of Sigma-Aldrich Co. LLC_x000D_ Biochem/physiol Actions_x000D_ Ras and Rab interactor 3 (RIN3) plays an essential role in vesicular trafficking by interacting with small GTPases such as Ras and Rab14. In addition, it also interacts with amphiphysin II and promotes receptor-mediated endocytosis. Mutations in the gene increases the risk of susceptibility to Paget’s disease of bone (PDB). The encoded protein acts as a guanine nucleotide exchange factor (GEF) for Rab 5 and Rab31. RIN3 plays an important role in basic mast cell functions and pathologies such as mast cell mediated chronic inflammation and mast cell hyperproliferation by regulating receptor tyrosine kinase (KIT) activity._x000D_ Features and Benefits_x000D_ Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section._x000D_ Every Prestige Antibody is tested in the following ways:_x000D_ • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues._x000D_ • Protein array of 364 human recombinant protein fragments._x000D_ • Validated for multiple commonly used applications such as IHC (Immunohistochemistry), IF (Immunofluorescence), and WB (Western Blot)_x000D_ Linkage_x000D_ Corresponding Antigen APREST79570.