Description_x000D_ General description_x000D_ Purified mouse monoclonal antibody. Recognizes the ~18 kDa SKP1A protein._x000D_ Recognizes the ~18 kDa SKP1A protein in HeLa cells._x000D_ This Anti-SKP1A Mouse mAb (1H8) is validated for use in Immunoblotting for the detection of SKP1A._x000D_ Immunogen_x000D_ A recombinant polypeptide corresponding to amino acids of 53-161 human SKP1A, expressed as a GST fusion protein_x000D_ Application_x000D_ _x000D_ _x000D_ Immunoblotting (1-5 µg/ml_x000D_ Immunocytochemistry (10 µg/ml)_x000D_ Physical form_x000D_ In PBS, pH 7.2._x000D_ Reconstitution_x000D_ Following initial thaw, aliquot and freeze (-20°C)._x000D_ Warning_x000D_ Toxicity: Regulatory Review (Z)_x000D_ Analysis Note_x000D_ Negative Control_x000D_ 293T cells_x000D_ Positive Control_x000D_ HeLa cells_x000D_ Other Notes_x000D_ Variables associated with assay conditions will dictate the proper working dilution.