Description_x000D_ General description_x000D_ Transcription factor SOX-17 (UniProt: Q61473; also known as SOX-17) is encoded by the Sox-17 gene (Gene ID: 20671) in murine species. Sox17 protein is a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. It binds to the sequences 5′-AACAAT-3 or 5′-AACAAAG-3′ and modulates transcriptional regulation via WNT3A. Sox17 is reported to act upstream of the Notch system and downstream of the canonical Wnt system and acts as a link between the canonical Wnt and Notch signaling systems. It is involved in the regulation of the formation of cells that go on to develop into endodermal tissues like pancreatic and liver cells. SOX17 is the key regulator of human primordial germ cells (hPGSc) fate and is considered as one of the earliest markers. It is also shown to be the transcription factor for the regulation of oligodendrocyte progenitor cells, maintenance of fetal hematopoeitic stem cells, and in the mediation of cardiac muscle cell formation. SOX17 is selectively expressed in arterial endothelial cells at the early stages of embryonic development and maintains the same distribution postnatally and in the adult. Inactivation of SOX17 in endothelial cells in mouse embryo leads to a lack of arterial differentiation and vascular remodeling, which that results in embryo death in utero. (Ref.: Corada, M., et al. (2013). Nat. Communications 4, 2609; Niakan, KK et al. (2010). Gene Dev. 24(3): 312-326)._x000D_ Specificity_x000D_ This polyclonal antibody detects Sox17 in human, mouse, and rat. It targets an epitope within 17 amino acids from the N-terminal region._x000D_ Immunogen_x000D_ KLH-conjugated linear peptide corresponding to 17 amino acids from the N-terminal region of murine Sox17._x000D_ Application_x000D_ Anti-SOX17, Cat. No. 09-038-I, is a highly specific rabbit polyclonal antibody that targets Transcription factor SOX-17 and has been tested in Immunohistochemistry (Paraffin), Immunofluorescence, and Western Blotting._x000D_ Anti-SOX17, Cat. No. 09-038-I-25UG, is a highly specific rabbit polyclonal antibody, that targets Transcription factor SOX-17 and has been tested in Western Blotting and Immunofluorescence and Immunohistochemistry (Paraffin)._x000D_ Research Category_x000D_ Neuroscience_x000D_ Western Blotting Analysis: 2 µg/mL from a representative lot detected SOX17 in 10 µg of human spinal cord tissue lysate._x000D_ Immunofluorescence Analysis: A 1:150-300 dilution from a representative lot detected SOX17 in human testis and rat colon tissues._x000D_ Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected SOX17 in human prostate, mouse stomach, and rat uterus tissues._x000D_ Western Blotting Analysis: 2 µg/mL from a representative lot detected SOX17 in 10 µg of human spinal cord tissue lysate._x000D_ Immunofluorescence Analysis: A 1:150-300 dilution from a representative lot detected SOX17 in human testis and rat colon tissues._x000D_ Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected SOX17 in human prostate, mouse stomach, and rat uterus tissues._x000D_ Target description_x000D_ ~40 kDa observed; 44.65 kDa calculated. Uncharacterized bands may be observed in some lysate(s)._x000D_ Physical form_x000D_ Affinity Purified_x000D_ Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide._x000D_ Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide._x000D_ Storage and Stability_x000D_ Stable for 1 year at 2-8°C from date of receipt._x000D_ Stable for 1 year at 2-8°C from date of receipt._x000D_ Disclaimer_x000D_ Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals._x000D_ Quality_x000D_ Evaluated by Western Blotting in H9 human stem cells._x000D_ Western Blotting Analysis: 2 µg/mL of this antibody detected SOX17 in 10 µg of H9 human stem cells lysate._x000D_ Linkage_x000D_ Replaces: 09-038_x000D_ Other Notes_x000D_ Concentration: Please refer to lot specific datasheet.