Description General description Immunoaffinity purified rabbit polyclonal antibody. Recognizes the synapsin I protein. Recognizes the synapsin I protein in rat brain. This Anti-Synapsin I Rabbit pAb is validated for use in ELISA, Immunoblotting Immunocytochemistry Immunoprecipitation for the detection of Synapsin I. Immunogen Bovine purified, brovine brain synapsin I Application ELISA (1:2500-1:10,000) Frozen Sections (see application references) Immunoblotting (1:200) Immunocytochemistry (1:500-1:2500) Immunoprecipitation (1 µg/200 µg lysate) Physical form Lyophilized from 5 mM ammonium bicarbonate. Reconstitution Reconstitute with 50 µl PBS. Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions. Packaging 10 μg in Plastic ampoule Warning Toxicity: Standard Handling (A) Analysis Note Positive Control Rat brain Other Notes Stone, L.M., et al. 1994. J. Neurosci.14, 301. Mandell, J.W., et al. 1992. J. Neurosci.12, 1736. Fletcher, T.L., et al. 1991. J. Neurosci.11, 1617. Moore, R.Y., and Berstein, M. 1989. J. Neurosci.9, 2151. This antibody is specific for synapsin I and can be used to estimate synaptic density and synaptogenesis. Strong and specific immunolabeling of the synapsin I doublet seen in immunoblotting at a 1:200 dilution. This immunolabeling can be blocked by preadsorption of antibody with synapsin I. Variables associated with assay conditions will dictate the proper working dilution.