Description General description Mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS1 mouse myeloma cells (see application references). Recognizes both the ~27 kDa glycosylated and the ~24 kDa unglycosylated forms of TIMP-3. Recognizes the ~27 kDa glycosylated and the ~24 kDa non-glycosylated forms of TIMP-3. Does not cross-react with TIMP-1 or TIMP-2 in CaCO-2 and HEK293 cell and eye tissue. This Anti-TIMP-3 Mouse mAb (136-13H4) is validated for use in Frozen sections, Immunoblotting, Immunofluorescence, Immunoprecipitation, Paraffin sections for the detection of TIMP-3. Immunogen Human a synthetic peptide corresponding to amino acids 170-188 of human TIMP-3 Application Frozen sections (2-10 µg/ml) Immunoblotting (5 µg/ml) Immunofluorescence (not recommended) Immunoprecipitation (not recommended) Paraffin sections (10 µg/ml, heat pre-treatment required, see application references) Physical form In 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0. Reconstitution Following initial thaw, aliquot and freeze (-20°C). Legal Information Manufactured by Daiichi Fine Chemical Co., Ltd. Not available for sale in Japan. Packaging 100 μg in Plastic ampoule Please refer to vial label for lot-specific concentration. Warning Toxicity: Standard Handling (A) Analysis Note Positive Control 293 or Caco-2 cells or unfixed eye tissue Other Notes Anand-Apte, B., et al. 1997. Invest. Ophthalmol. Vis. Sci.38, 817. Butler, G.S., et al. 1997. Eur. J. Biochem.244, 653. Fariss, R.N., et al. 1997. Am. J. Pathol.150, 323. Vranka, J.A., et al. 1997. Curr. Eye Res.16, 102. Ruiz, A., et al. 1996. Biochem. Biophys. Res. Commun.226, 467. Kishnani, N.S., et al. 1995. Matrix Biol.14, 479. Cottam, D.W. and Rees, R.C. 1993. Intl J. Oncol.2, 861. Stetler-Stevenson, W. G., et al. 1993. FASEB J.7, 1434. Woessner, J.F. 1991. FASEB J.5, 2145. Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Seminars in Cancer Biology, ed. M. M. Gottesman. Vol. 1(2), 99.