Description_x000D_ General description_x000D_ Purified mouse monoclonal antibody. Recognizes the ~12-16 kDa TOMM20 protein._x000D_ Recognizes the ~12-16 kDa TOMM20 protein in HeLa, PC12, and NIH3T3 cells and human small intestine tissue._x000D_ This Anti-TOMM20 Mouse mAb (4F3) is validated for use in ELISA, Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of TOMM20._x000D_ Immunogen_x000D_ Full-length, recombinant, human TOMM20 (aa 1-146), expressed as a GST fusion protein_x000D_ Application_x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ ELISA (see coments)_x000D_ Immunoblotting (1-5 µg/ml)_x000D_ Immunocytochemistry (10 µg/ml)_x000D_ Paraffin Sections (3 µg/ml)_x000D_ Physical form_x000D_ In PBS, pH 7.2._x000D_ Reconstitution_x000D_ Following initial thaw, aliquot and freeze (-20°C)._x000D_ Warning_x000D_ Toxicity: Regulatory Review (Z)_x000D_ Analysis Note_x000D_ Negative Control_x000D_ 293T cells_x000D_ Positive Control_x000D_ HeLa cells, PC12 cells, NIH3T3 cells, Human small intestine tissue_x000D_ Other Notes_x000D_ For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.