Description General description Rabbit polyclonal antibdy supplied as undiluted serum that has been adsorbed against GST to remove GST-reactive antibodies. Recognizes the ~45 kDa TRB3 protein. Recognizes the ~45 kDa TRB3 protein in HepG2 cells. TRB3 is a protein that is reported to disrupt insulin signaling by binding directly to Akt and blocking activation of the kinase. This Anti-TRB3 (1-145) Rabbit pAb is validated for use in Immunoblotting, Immunocytochemistry, Immunoprecipitation for the detection of TRB3 (1-145). Immunogen Mouse a recombinant protein consisting of amino acids 1-145 of mouse TRB3, fused to GST Application Immunoblotting (1:2500) Immunocytochemistry (1:2500) Immunoprecipitation (1:250) Physical form Undiluted serum. Reconstitution For long-term storage aliquot and freeze (-20°C). Avoid freeze/thaw cycles. Packaging 100 μL in Plastic ampoule Warning Toxicity: Standard Handling (A) Analysis Note Positive Control HepG2 cells Other Notes Antibody should be titrated for optimal results in individual systems. Du, K., et al. 2003. Science300, 1574.