Description General description Purified rabbit polyclonal antibody. Recognizes the ~35 kDa monomeric tryptase subunits (reducing conditions). Recognizes the ~35 kDa tryptase protein. This Anti-Tryptase Rabbit pAb is validated for use in Immunoblotting, ELISA for the detection of Tryptase. Immunogen Human Lung purified, human lung tryptase Application Immunoblotting (1:500-1:1000) ELISA (see comments) Physical form In 0.2 µm sterile-filtered PBS, pH 7.4. Reconstitution Following initial thaw, aliquot and freeze (-20°). Packaging 500 μg in Plastic ampoule Warning Toxicity: Standard Handling (A) Other Notes Also reported to work for ELISA. Variables associated with assay conditions will dictate the proper working dilution. He, S., et al. 1998. J. Pharmacol. Exp. Ther.286, 289. Molino, M., et al. 1997. J. Biol. Chem.272, 4043.