Description_x000D_ General description_x000D_ Tyrosine hydroxylase (TH) is encoded by the gene mapped to human chromosome 11p15. TH is a tetramer of four identical subunits, which is characterized with a regulatory, catalytic, and tetramerization domains. The enzyme utilizes tyrosine, tetrahydrobiopterin (BH4) and O2 as cosubstrates, and Fe2+ as a cofactor._x000D_ Immunogen_x000D_ SDS-denatured rat tyrosine hydroxylase purified from pheochromocytoma._x000D_ Application_x000D_ Anti-tyrosine hydroxylase antibody produced in rabbit has been used in western blot analysis and immunohistochemistry._x000D_ Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below._x000D_ Immunofluorescence (1 paper)_x000D_ Immunohistochemistry (1 paper)_x000D_ Target description_x000D_ Tyrosine hydroxylase (TH) is involved in the conversion of phenylalanine to dopamine. It catalyzes the initial, rate-limiting step of the catecholamine biosynthetic pathway._x000D_ Physical form_x000D_ Solution in 100 μL in 10 mM HEPES, pH 7.5, 150 mM NaCl, 100 μg/mL BSA and 50% glycerol._x000D_ Disclaimer_x000D_ Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals._x000D_ Biochem/physiol Actions_x000D_ Tyrosine hydroxylase (TH) catalyzes the first rate limiting step in the biosynthesis of catecholamine neurotransmitter i.e. the conversion of L-tyrosine to L-dopa. Inhibition of TH by L-phenylalanine, might play a crucial role in phenylketonuria and block the synthesis of norepinephrine. Activity of TH can be regulated by phosphorylation. Decreased expression of TH is associated with various neuropsychiatric diseases such as schizophrenia and Parkinson’s disease (PD).