Description General description Protein A purified rabbit polyclonal antibody. Recognizes the ~34-50 kDa isoforms of VEGF. Recognizes the ~34-50 kDa isoforms of VEGF. Cat. No. PC315 is recommended for immunohistochemical staining of rodent tissues. Recognizes VEGF in human tissues by immunoblotting but not by immunohistochemistry. Immunogen Human recombinant, human VEGF₁₆₅ Application Paraffin Sections (1:200-1:400, pressure cooker pre-treatment required, see comments) Physical form In 10 mM PBS, 0.2% BSA, pH 7.6. Reconstitution Following intitial thaw, aliquot and freeze (-20°C). Packaging 500 μg in Plastic ampoule Please refer to vial label for lot-specific concentration. Warning Toxicity: Standard Handling (A) Analysis Note Positive Control Recombinant VEGF or blood vessels in skin or intestine (rodent only) Other Notes Soker, S., et al. 1998. Cell92, 735. Kroll, J. and Waltenberger, J. 1997. J. Biol. Chem.272, 32521. Klagsbrun, M. and Soker, S. 1993. Current Biology3, 699. Senger, D., et al. 1993. Cancer and Metastasis Rev.12, 303. Brown, L., et al. 1992. Kidney International42, 1457. Dvorak, H., et al. 1991. J. Exp. Med.174, 1275. Tested on formalin-fixed, paraffin embedded tissue. Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 8.0, for 10-20 min followed by cooling at RT for 20 min. Antibody should be titrated for optimal results in individual systems.