Description General description A reversible protease inhibitor produced by actinomycetes. Inhibits cathepsin A and B as well as papain, and trypsin-like serine proteases. Acts by forming a hemiacetal adduct between the aldehyde group of the inhibitor and the active proteinase. Effective at concentrations range from 1 to 100 µM. Effective concentration: 1-100 µM. Peptidyl arginine aldehyde protease inhibitor produced by actinomycetes. Inhibitor of Ca2+-dependent endopeptidases. Has specificity similar to Leupeptin (Cat. No. 108975). Inhibits trypsin-like serine proteases, papain and some cysteine proteases (IC50 = 300 µM). Packaging 10 mg in Plastic ampoule Biochem/physiol Actions Cell permeable: no Primary Target trypsin-like serine proteases, papain, and some cysteine proteases Product does not compete with ATP. Reversible: yes Target IC50: 300 µM against trypsin-like serine proteases, papain and some cysteine proteases; Effective concentration: 1-100 µM Warning Toxicity: Standard Handling (A) Reconstitution Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 1 week at 4°C or for up to 1 month at -20°C. Other Notes Lyupina, Y.V., et al. 1996. Eur. J. Pharmacol. 304, 23. Ichikawa, S., et al. 1985. J. Reprod. Fertil.73, 385 Umezawa, H. 1976. Methods Enzymol.55, 678. Suda, H., et al. 1972. J. Antibiot. (Tokyo) 25, 263.