Description General description This product has been discontinued. General proteolytic substrate. Provides a rapid, simple method for the detection of proteases in aqueous solutions. Color yield (520 nm): >1.0; blank (520 nm, 50 mg/5 ml buffer, 10 minutes at 37°C, pH 7.0): <0.2. Mesh: <50 (minimum 80%). General proteolytic substrate. Provides a rapid and simple spectrophotometric method to detect the presence of proteases in aqueous solutions. As the insoluble substrate is cleaved by proteases, it releases a red dye into the surrounding buffer. Incubation of 50 mg AZOCOLL Substrate with 2 units of PRONASE Protease for 15 min yields an absorbance of greater than 1.0 at 520 nm. Packaging 1 g in Plastic ampoule Warning Toxicity: Standard Handling (A) Other Notes Poilane, I., et al. 1998. Can. J. Microbiol. 44, 157. Braganza, V.J., and Simmons, W.H. 1991. Biochemistry 30, 4997. Moore, G.L. 1969. Anal. Biochem. 32, 122.