Description_x000D_ General description_x000D_ Bilirubin, also known as hematoidin, is a degradation product formed as a result of heme catabolism in the liver. Bilirubin circulates in the blood stream as either the unconjugated insoluble form (indirect bilirubin) or the soluble glucuronide-conjugated form (direct bilirubin). Conjugated bilirubin moves from the bile canaliculi of the liver to the gall bladder where it is excreted into the small intestine during digestion. High levels of bilirubin can result in jaundice and may indicate liver disease, blood disorders, or blockage of the bile ducts._x000D_ Application_x000D_ Bilirubin Assay Kit has been used to measure the total bilirubin concentration in samples._x000D_ Features and Benefits_x000D_ Compatible with high-throughput handling systems. Can be adapted for use with cuvettes._x000D_ Suitability_x000D_ Suitable for the detection of of both total and conjugated bilirubin in serum and plasma samples._x000D_ Principle_x000D_ This assay, based on the Jendrassik-Grof method, utilizes the reaction of bilirubin with diazotized sulfanilic acid resulting in a colorimetric product measured at 530 nm, proportionate to the bilirubin present in the sample. This assay kit measures both total and conjugated bilirubin.