Description_x000D_ General description_x000D_ PharmaGrade products are manufactured under appropriate controls for use as a raw material in pharma or biopharmaceutical production. For more information on the PharmaGrade Program, visit sigma-aldrich.com/safc-pharmagrade._x000D_ Packaging_x000D_ 1 kg in poly bottle_x000D_ 10 kg in poly drum_x000D_ 100 g in poly bottle_x000D_ Application_x000D_ Bis Tris Propane is a unique biological buffer also referred to as a “Universal” buffer, which means the buffer capacity covers a wide range. Bis Tris Propane has two pKas of 6.3 and 9.5, which expands the buffering range from 5.0-10.5-making this a versatile buffer for biological systems. Bis Tris Propane is considered to be non-toxic to culture cell lines and provides high-solution clarity._x000D_ A review of DNA polymerase techniques lists bis tris propane as a suitable buffer for PCR methods as a universal buffer pH gradient is employed._x000D_ Linkage_x000D_ Enhanced Quality Product Documentation on PharmaGrade BIS-TRIS Propane