Description_x000D_ Application_x000D_ Bovine serum albumin has been used-_x000D_ • along with BTS (Beltsville thawing solution), for the extension of sperms during sperm motion analysis_x000D_ • as a component of modified Krebs-Ringer buffer (dmKRBT)_x000D_ • in gel shift analysis_x000D_ Packaging_x000D_ 1, 5 kg in poly drum_x000D_ 10, 50, 100, 500 g in poly bottle_x000D_ Biochem/physiol Actions_x000D_ Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies._x000D_ Preparation Note_x000D_ Often referred to as Cohn fraction V; this product is prepared by a modified method of the Cohn cold ethanol fractionation method._x000D_ Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.