Description_x000D_ Application_x000D_ Useful for kinase assay and Western blot._x000D_ Biochem/physiol Actions_x000D_ Recombinant full-length human BTK (C481S) was expressed by Baculovirus in Sf9 insect cells using an N-terminal His tag. BTK (also known as Bruton tyrosine kinase) plays a crucial role in B-lymphocyte differentiation and activation. BTK interacts with SRC homology 3 domains of FYN, LYN and HCK that are activated upon stimulation of B- and T-cell receptors (1).Defects in the BTK gene cause Agammaglobulinemia, an X-linked immunodeficiency characterized by failure to produce mature B lymphocyte cells and associated with a failure of Ig heavy chain rearrangement. The unique role of BTK makes it a desirable target for potential anti-cancer, anti-inflammatory and anti-viral agents as well as other treatments (2)._x000D_ Reconstitution_x000D_ Formulated in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, with 25% glycerol._x000D_ Storage and Stability_x000D_ Store in aliquots at -70°C. Avoid freeze/thaw cycles._x000D_ Legal Information_x000D_ PRECISIO is a registered trademark of Sigma-Aldrich Co. LLC