Description General description A potent inhibitor of Ca2+-stimulated myosin S1 ATPase (IC50 = ~ 5 µM) and reversibly blocks the gliding motility. It also weakens myosin’s interaction with F-actin. Much less effective in suppressing contraction in rat myocardial or rabbit slow twitch muscle and has no effect on platelet myosin II. An aryl sulphonamide that acts as a potent inhibitor of Ca2+-stimulated myosin S1 actin-stimulated ATPase activity (IC50 = ~5 µM). Also blocks actin-stimulated ATPase activity with similar potency (IC50 = 5 µM). Reversibly blocks gliding motility of skeletal muscle myosin (IC50< 2 µM). Less effective in suppressing contraction in rat myocardial and rabbit slow twitch muscle compared to skeletal muscle. Does not inhibit platelet myosin II. Packaging 5 mg in Plastic ampoule Biochem/physiol Actions Cell permeable: no Primary Target Ca2+-stimulated myosin S1 ATPase Product does not compete with ATP. Reversible: yes Target IC50: ~5 µM against Ca2+-stimulated myosin S1 ATPase Packaging Packaged under inert gas Warning Toxicity: Standard Handling (A) Reconstitution Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Other Notes Cheung, A., et al. 2002. Nat. Cell Biol.4, 83.