Description; General description; Calcein is useful in fitting soft and hybrid contact lenses, along with its evaluation. It has a molecular weight of 710kDa and is generally preferred over fluorescein. Calcein is known to stain devitalized tissue. It is not preferred for highly hydrated soft lenses.; Application; Calcein has been used: • in whole cell staining • in histomorphometry and micro-computed tomography • to label active bone-forming surfaces; Packaging; 5, 10, 25 g in glass bottle; Biochem/physiol Actions; Calcein is a calcium-dependent fluorescent molecule. It is mainly required to study bone metabolism under in vivo conditions and helps in the staining of pit area under in vitro conditions. Calcein is used for the fluorometric determination of calcium and EDTA (ethylenediaminetetraacetic acid) titration of calcium.