Description General description An internally quenched peptide substrate that is optimized for calpain-1 amino acid recognition motifs at the primed as well as the non-primed sides. Shown to be kinetically superior, in terms of turnover rate, to Calpain Substrate III, Fluorogenic (Cat. No. 208771), Calpain-1 Substrate, Fluorogenic (Cat. No. 208748), and Calpain Substrate II, Fluorogenic (Cat. No. 208731) by ~8-, 18-, and 310-fold, respectively. Excitation: ~335 nm; emission: ~505 nm. An internally quenched peptide substrate that is optimized for calpain-1 amino acid recognition motifs at the primed as well as the non-primed sides. Shown to be kinetically superior, in terms of turnover rate, to Calpain Substrate III, Fluorogenic (Cat. No. 208771), Calpain-1 Substrate, Fluorogenic (Cat. No. 208748), and Calpain Substrate II, Fluorogenic (Cat. No. 208731) by ~8-, 18-, and 310-fold, respectively. Packaging 1 mg in Glass bottle Biochem/physiol Actions Cell permeable: no Primary Target Calpain-1 Substrate Product does not compete with ATP. Reversible: no Packaging Packaged under inert gas Warning Toxicity: Irritant (B) Sequence H₂N-Glu(EDANS)-Pro-Leu-Phe~Ala-Glu-Arg-Lys(DABCYL)-CO₂H Physical form Supplied as a trifluoroacetate salt. Reconstitution Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C. Other Notes Cuerrier, D., et al. 2007. J. Biol. Chem.282, 9600. Cuerrier, D., et al. 2005. J. Biol. Chem.280, 40632.