Description General description Fluorogenic ICE substrate. Reaction can be monitored by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore. AFC (7-amino-4-trifluoromethylcoumarin) based fluorometric substrates are superior to AMC (7-amino-4-methylcoumarin) substrates because released AFC has a greater Stoke′s shift than AMC. Excitation/emission maxima (nm) for AFC and AMC are 400/505 and 380/460, respectively. Fluorogenic caspase-1 substrate. Reaction can be monitored visually or quantitatively by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore. Packaging 5 mg in Plastic ampoule Biochem/physiol Actions Cell permeable: no Primary Target Fluorogenic caspase-1 substrate Product does not compete with ATP. Reversible: no Warning Toxicity: Standard Handling (A) Sequence Ac-Tyr-Val-Ala-Asp-AFC Reconstitution Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C. Other Notes Jones, R.A., et al. 1998. Hepatology 27, 1632. Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312. Walker, N.P., et al. 1994. Cell 78, 343. Thornberry, N.A., et al. 1992. Nature 356, 768.