Description General description A potent, cell-permeable and irreversible inhibitor of CPP-32/apopain, a member of the ICE/CED-3 family of cysteine proteases. A potent, cell-permeable, and irreversible inhibitor of caspase-3 as well as caspase-6, caspase-7, caspase-8, and caspase-10. When using with purified native or recombinant enzyme, pretreatment with an esterase is required. A 5 mM (250 µg/75 µl) solution of Z-DEVD-FMK (Cat. No. 264156) in DMSO is also available. Packaging 1 mg in Plastic ampoule 250 μg in Plastic ampoule Biochem/physiol Actions Cell permeable: yes Primary Target caspase-3, caspase-6, caspase-7, caspase-8, caspase-10 Product does not compete with ATP. Reversible: no Warning Toxicity: Standard Handling (A) Sequence Z-Asp(OCH₃)-Glu(OCH₃)-Val-Asp(OCH₃)-FMK Reconstitution Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C. Other Notes Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312. Masuda, Y., et al. 1997. Biochem. Biophys. Res. Commun. 234, 641. Nicholson, D.W. 1996. Nature Biotech. 14, 297. Schlegel, J., et al. 1996. J. Biol. Chem. 271, 1841. Nicholson, D.W., et al. 1995. Nature 376, 37.; Description