Description General description Note: 1 mU = 1 milliunit. Recombinant, human cathepsin S expressed in E. coli without a tag or fusion protein. A major lysosomal cysteine proteinase preferentially expressed in macrophages and microglia. Exhibits high specific activity. Recombinant, human cathepsin S expressed in E. coli without a tag or fusion protein. A major lysosomal cysteine proteinase that is preferentially expressed in macrophages and microglia. Shown to be involved in the processing of antigenic peptides for presentation by MHC Class II molecules on the surface of antigen-presenting cells by mediating invariant (Ii) chain degradation. Inhibition of cathepsin S results in impaired antigen-presentation. Packaging 25 μg in Plastic ampoule Warning Toxicity: Harmful (C) Unit Definition One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol Z-VVR-AMC per min at 37°C, pH 6.5. Note: 1 mU = 1 milliunit. Physical form In 35 mM potassium phosphate, 35 mM Sodium acetate, 2 mM DTT, 2 mM EDTA, 50% ethylene glycol, pH 6.5. Reconstitution Following initial thaw, aliquot and freeze (-70°C). Other Notes Liuzzo, J.P., et al. 1999. Mol. Med.5, 334. Riese, R.J., et al. 1998. J. Clin. Invest.101, 2351. Sukhova, G.K., et al. 1998. J. Clin. Invest.102, 576. Kirschke, H., and Wiederanders, B. 1994. Methods Enzymol.244, 500. Xin, X.Q., et al. 1992. Arch. Biochem. Biophys.299, 334. Kirschke, H., et al. 1989. Biochem. J.264, 467.