Description; Application; CelLytic™ B Cell Lysis Reagent has been used for cell lysis for the extraction of coenzyme Q10,[1] recombinant Src kinase associated phosphoprotein 1- glutathione S-transferases (GST) fusion protein[2] and human α-synuclein.[3]; A proprietary, non-denaturing formulation of zwitterionic detergents used for the lysis of bacterial cells and extraction of recombinant proteins.; Features and Benefits; • Higher protein extraction efficiency than traditional methods such as sonication and lysozyme • Scalable for 1 to 25 grams of bacterial cell paste • No interference with downstream applications such as affinity chromatography, IP, and Western blotting • Compatible with protease inhibitors, inhibitor cocktails, chaotropes, salts, chelating agents and reducing agents; Other Notes; Detergent blend formulated in 40 mM Trizma® HCl (pH 8.0).; Legal Information; Covered by US Patent No 7,282,475 B2 and are sold for research use only. Commercial use requires addtional licenses.; CelLytic is a trademark of Sigma-Aldrich Co. LLC; Trizma is a registered trademark of Sigma-Aldrich Co. LLC